Study record
MOTS-c preserves mitochondrial subpopulation bioenergetics and genome integrity to attenuate cardiac ischemia reperfusion injury
Santhanam SS, Jayaraman S, Rajesh SS et al. · Mol Biol Rep · 2026
What this study reported
Reported that ischemia-reperfusion impaired mechanical recovery, increased oxidative stress and lowered mitochondrial enzyme activities, membrane potential, mtDNA copy number and mitochondrial gene expression in isolated rat hearts. Reported that MOTS-c added before ischemia or at reperfusion was associated with better post-ischemic mechanical recovery and partial preservation of these mitochondrial measures, with responses varying across parameters.
Limitations
Very small group sizes; isolated female rat hearts without systemic circulation or blood components; supraphysiological peptide exposure in the perfusate and no mechanistic validation.
Study record
| Study model | Tissue / sample study (ex vivo) |
|---|---|
| Substance studied | MOTS-c administeredSynthetic MOTS-c was given to cells, animals or people. |
| Species | rat |
| System studied | Isolated female Wistar rat hearts on a Langendorff perfusion system with global ischemia and reperfusion |
| Population | Not reported |
| Design | Controlled ex vivo perfused heart experiment |
| Sample size as reported | 6 hearts per group |
| Control group | ischemia-reperfusion hearts without MOTS-c |
| Route (data field only) | other |
| Endpoints measured | cardiac mechanical recovery; myocardial injury markers; mitochondrial bioenergetics in subsarcolemmal and interfibrillar populations; oxidative stress; mtDNA copy number; mitochondrial regulatory gene expression |
| Pathways measured | Oxidative stress · SIRT1 / PGC-1α / biogenesis |
| Research areas | Cardiovascular · Mitochondrial biology |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Mammalian
- Sample size
- Small
- Controls
- Appropriate
- Outcome validity
- Surrogate
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.