Study record
Diagnostic relevance of Humanin, GAS5 and miR-21/miR-103 in prostate disease risk stratification
Coradduzza D, Cruciani S, Sibono L et al. · Clin Exp Med · 2025
What this study reported
Measured circulating humanin and MOTS-c, GAS5 and exosomal miR-21 and miR-103 in men investigated for prostate disease. Found lower humanin and GAS5 in precancerous lesions and prostate cancer than in benign prostatic hyperplasia, higher exosomal miR-21 and miR-103 in prostate cancer, and higher MOTS-c in precancerous lesions than in benign prostatic hyperplasia. Reported that a plasma model combining humanin, GAS5 and MOTS-c reached 95% cross-validated classification accuracy across these clinical groups.
Limitations
Single cross-sectional cohort with no external validation, and near-perfect reported AUCs raise the possibility of overfitting; group sizes are not given, men only by disease definition, and MOTS-c is one of several biomarkers rather than the primary focus.
Study record
| Study model | Human observational |
|---|---|
| Substance studied | SeveralMore than one of these is central to the paper (common for reviews). |
| Species | human |
| System studied | Male patients investigated for suspected prostate cancer, grouped as benign hyperplasia, precancerous lesions or prostate cancer |
| Population | men with suspected prostate cancer, classified as benign prostatic hyperplasia, precancerous lesions or prostate cancer |
| Design | Cross-sectional diagnostic biomarker study |
| Sample size as reported | 375 male patients |
| Control group | patients with benign prostatic hyperplasia as the comparison group |
| Route (data field only) | Not reported |
| Endpoints measured | plasma MOTS-c; plasma humanin; GAS5; exosomal miR-21 and miR-103; ROC/AUC discrimination; classification accuracy |
| Pathways measured | Apoptosis · Inflammation |
| Research areas | Cancer · Methods |
| Evidence tier | B — Other human study |
| Publication types | Journal Article |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Indirect
- Model applicability
- Human
- Sample size
- Adequate
- Controls
- Limited
- Outcome validity
- Exploratory
- Support for its own conclusion
- Weak
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.