Study record
The impact of mitokine MOTS-c administration on the soleus muscle of rats subjected to a 7-day hindlimb suspension
Sidorenko DA, Lvova ID, Tyganov SA et al. · J Muscle Res Cell Motil · 2025
What this study reported
Reported that administered MOTS-c prevented the increase in soleus muscle fatigue and the transformation of slow fibres into fast fibres during hindlimb unloading in male rats. Found that atrophy of slow fibres, but not fast fibres, was mitigated, that the decreases in Akt and GSK3β phosphorylation were prevented, that 18S and 28S rRNA remained at control levels, and that MuRF and Atrogin-1 mRNA were lower than in placebo-injected unloaded rats. Reported that decreases in several mitochondrial biogenesis markers and in ACC phosphorylation were also prevented in these rats.
Limitations
Male rats only and a single strain; group sizes not reported in the abstract; a single unloading model with surrogate molecular endpoints and no functional recovery follow-up.
Study record
| Study model | Animal study (in vivo) |
|---|---|
| Substance studied | MOTS-c administeredSynthetic MOTS-c was given to cells, animals or people. |
| Species | rat |
| System studied | Male Wistar rats undergoing hindlimb unloading, with soleus muscle analysed |
| Population | Not reported |
| Design | Controlled animal experiment |
| Sample size as reported | not reported |
| Control group | placebo-injected hindlimb-unloaded rats and non-unloaded control rats |
| Route (data field only) | intraperitoneal |
| Endpoints measured | muscle fatigue; slow-to-fast fibre transformation; fibre cross-sectional area; Akt and GSK3β phosphorylation; MuRF and Atrogin-1 mRNA; mitochondrial biogenesis markers and ACC phosphorylation |
| Pathways measured | AKT / mTOR / FOXO · AMPK signalling · SIRT1 / PGC-1α / biogenesis |
| Research areas | Skeletal muscle · Mitochondrial biology |
| Evidence tier | C — Animal in vivo |
| Publication types | Journal Article, Research Support, Non-U.S. Gov't |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Mammalian
- Sample size
- Not reported
- Controls
- Appropriate
- Outcome validity
- Surrogate
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.