Study record
MOTS-c Promotes Glycolysis via AMPK-HIF-1α-PFKFB3 Pathway to Ameliorate Cardiopulmonary Bypass-induced Lung Injury
Shen Z, Lu P, Jin W et al. · Am J Respir Cell Mol Biol · 2025
What this study reported
Reported lower plasma MOTS-c concentrations in patients who developed acute lung injury after cardiopulmonary bypass. Found that MOTS-c pretreatment was associated with greater glycolytic flux, restored PFKFB3 expression, lower oxidative stress and less ferroptosis in a mouse lung ischemia-reperfusion model and in pulmonary microvascular endothelial cells, with higher phosphorylated AMPKα and HIF-1α; genetic and pharmacological PFKFB3 inhibition abolished these differences in cells.
Limitations
Single-centre surgical cohort with an observational biomarker component only; mechanistic work confined to one mouse injury model and one endothelial cell type; no human exposure to MOTS-c was tested.
Study record
| Study model | Human observational |
|---|---|
| Substance studied | SeveralMore than one of these is central to the paper (common for reviews). |
| Species | human, mouse |
| System studied | Patients undergoing cardiopulmonary bypass; mouse lung ischemia-reperfusion model; pulmonary microvascular endothelial cells |
| Population | Patients undergoing cardiopulmonary bypass during cardiac surgery, with and without acute lung injury |
| Design | Prospective controlled clinical observation combined with controlled animal and cell experiments |
| Sample size as reported | 107 patients undergoing cardiopulmonary bypass |
| Control group | Patients without acute lung injury; untreated injury model animals and cells |
| Route (data field only) | Not reported |
| Endpoints measured | plasma MOTS-c; inflammatory markers; glycolytic flux and PFKFB3 expression; oxidative stress and lipid peroxidation; ferroptosis markers; AMPKα and HIF-1α phosphorylation/expression |
| Pathways measured | AMPK signalling · Oxidative stress · Inflammation · Other |
| Research areas | Lung · Cardiovascular · Mitochondrial biology · Inflammation & immunity |
| Evidence tier | B — Other human study |
| Publication types | Journal Article, Research Support, Non-U.S. Gov't |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Human
- Sample size
- Adequate
- Controls
- Appropriate
- Outcome validity
- Surrogate
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.