Study record
MOTS-c Impact on Muscle Cell Differentiation and Metabolism Across Fiber Types
Leciejewska N, Pruszyńska-Oszmałek E, Kołodziejski P et al. · Cell Physiol Biochem · 2025
CellsMOTS-c administeredEvidence tier D
What this study reported
Reported that MOTS-c increased survival, ERK phosphorylation and muscle regulatory factor expression in C2C12 cells but not in L6 cells, reduced proliferation in both lines, and lowered lipid accumulation in C2C12 cells while increasing it in L6 cells.
Limitations
Two immortalised cell lines from different species rather than primary muscle; no in vivo confirmation; short exposure windows and no contractile or functional readouts.
Study record
| Study model | Cell study (in vitro) |
|---|---|
| Substance studied | MOTS-c administeredSynthetic MOTS-c was given to cells, animals or people. |
| Species | mouse, rat |
| System studied | C2C12 mouse and L6 rat muscle cell lines treated with MOTS-c and free fatty acids |
| Population | Not reported |
| Design | Cell culture experiments |
| Sample size as reported | Not reported |
| Control group | untreated cultures |
| Route (data field only) | in-culture |
| Endpoints measured | cell survival; proliferation; ERK phosphorylation; muscle regulatory factor expression; lipid accumulation |
| Pathways measured | MAPK signalling · Lipid metabolism |
| Research areas | Skeletal muscle · Metabolic |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Cellular
- Sample size
- Not reported
- Controls
- Appropriate
- Outcome validity
- Exploratory
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.