Study record
MOTS-c regulates pancreatic alpha and beta cell functions in vitro
Bień J, Pruszyńska-Oszmałek E, Kołodziejski P et al. · Histochem Cell Biol · 2024
CellsSeveralEvidence tier D
What this study reported
Reported that MOTS-c lowered insulin secretion and expression in INS-1E beta cells and increased glucagon secretion and expression in αTC-1 alpha cells, and that it affected cell viability and apoptosis. Also reported that insulin, glucagon, glucose and free fatty acids altered MOTS-c secretion in these cell lines.
Limitations
Immortalised cell lines from two species rather than primary islets; no in vivo confirmation; replicate numbers and exposure conditions are not given in the abstract.
Study record
| Study model | Cell study (in vitro) |
|---|---|
| Substance studied | SeveralMore than one of these is central to the paper (common for reviews). |
| Species | rat, mouse |
| System studied | INS-1E rat beta-cell and αTC-1 mouse alpha-cell lines exposed to MOTS-c, glucose and free fatty acids |
| Population | Not reported |
| Design | Cell culture experiments |
| Sample size as reported | Not reported |
| Control group | untreated cultures |
| Route (data field only) | in-culture |
| Endpoints measured | insulin secretion and expression; glucagon secretion and expression; MOTS-c secretion; cell viability; proliferation; apoptosis |
| Pathways measured | Insulin & glucose handling · Apoptosis |
| Research areas | Metabolic |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Cellular
- Sample size
- Not reported
- Controls
- Appropriate
- Outcome validity
- Exploratory
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.