Study record

Evidence of natural selection in the mitochondrial-derived peptides humanin and SHLP6

Gruschus JM, Morris DL, Tjandra N · Sci Rep · 2023

AnalyticalRelated mitochondrial peptideEvidence tier D
PubMed 37644144doi:10.1038/s41598-023-41053-0Full text (PMC)Compare with another study

What this study reported

Analysed vertebrate coding sequences of eight mitochondrial-derived peptides for synonymous codon bias as a signal of amino acid conservation. Reported strong codon bias and sequence conservation for humanin and SHLP6, no significant bias for SHLP1, SHLP2, SHLP3 and SHLP5, and no significant bias for MOTS-c and SHLP4 although both contained highly conserved N-terminal regions; also described a further candidate sequence preceding SHLP2, named SHLP2b.

Limitations

Computational sequence analysis with no experimental validation of peptide expression or function; inference of biological importance rests on codon-bias assumptions within overlapping RNA genes; restricted to vertebrate sequences, and MOTS-c is one of several peptides rather than the focus.

Study record

Annotated fields for this study
Study modelAnalytical / methods
Substance studiedRelated mitochondrial peptideThe paper is mainly about another mitochondrial-derived peptide, such as humanin.
SpeciesNot reported
System studiedVertebrate mitochondrial 12S and 16S rRNA gene sequences analysed for synonymous codon bias and conservation
PopulationNot reported
DesignComparative sequence and codon-bias analysis
Sample size as reportednot reported
Control groupNot reported
Route (data field only)Not reported
Endpoints measuredsynonymous codon bias; amino acid sequence conservation; identification of candidate peptide coding sequences
Pathways measuredNot reported
Research areasMitochondrial biology · Methods
Evidence tierD — Cell, tissue or analytical
Publication typesJournal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural

Appraisal

Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.

Relevance to MOTS-c
Indirect
Model applicability
n/a
Sample size
n/a
Controls
n/a
Outcome validity
Exploratory
Support for its own conclusion
Moderate

Provenance

  • Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
  • Annotated from the published abstract.
  • Editorial review: approved (2026-11-11).
  • Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.