Study record
Exogenous humanin and MOTS-c function as protective agents against gentamicin-induced hair cell damage
Waldmann D, Lu Y, Cortada M et al. · Biochem Biophys Res Commun · 2023
What this study reported
Determined expression of rattin, the humanin homolog, in inner ear tissues and tested humanin and MOTS-c in organ of Corti explants exposed to gentamicin. Reported a significant protective effect of both peptides on hair cells in these explants, with humanin treatment decreasing gentamicin-induced AKT phosphorylation and MOTS-c treatment increasing AMPKalpha phosphorylation in the explants.
Limitations
Ex vivo explant model only, with no in vivo hearing or functional outcomes; replicate numbers not reported in the abstract; MOTS-c examined alongside humanin with limited mechanistic dissection, and signalling readouts are single phosphorylation markers.
Study record
| Study model | Tissue / sample study (ex vivo) |
|---|---|
| Substance studied | SeveralMore than one of these is central to the paper (common for reviews). |
| Species | rat |
| System studied | Organ of Corti explants exposed to gentamicin; inner ear tissues examined for rattin expression |
| Population | Not reported |
| Design | Ex vivo explant culture experiments |
| Sample size as reported | not reported |
| Control group | gentamicin-exposed explants without peptide treatment |
| Route (data field only) | in-culture |
| Endpoints measured | hair cell survival; rattin expression in inner ear tissue; AKT phosphorylation; AMPKalpha phosphorylation |
| Pathways measured | AMPK signalling · AKT / mTOR / FOXO |
| Research areas | Nervous system · Mitochondrial biology |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Cellular
- Sample size
- Not reported
- Controls
- Limited
- Outcome validity
- Exploratory
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.