Study record
Β-GPA administration activates slow oxidative muscle signaling pathways and protects soleus muscle against the increased fatigue under 7-days of rat hindlimb suspension
Sharlo KA, Lvova ID, Sidorenko DA et al. · Arch Biochem Biophys · 2023
What this study reported
Measured slow-type muscle signalling in the unloaded rat soleus after hindlimb suspension with and without the creatine kinase inhibitor beta-guanidine propionic acid. Reported that the inhibitor preserved a slow-type signalling network including MOTS-C, AMPK, PGC-1alpha and microRNA-499, and that fatigue resistance, slow-type fibre percentage and mitochondrial DNA copy number were preserved in rats under muscle unloading.
Limitations
Small group size (8 per group); male rats only; single strain and a single 7-day unloading period; MOTS-C was one component of a signalling panel rather than the manipulated variable, so its contribution was not tested directly.
Study record
| Study model | Animal study (in vivo) |
|---|---|
| Substance studied | Endogenous MOTS-c measuredNaturally occurring MOTS-c was measured; nothing was given. |
| Species | rat |
| System studied | Male Wistar rats undergoing 7-day hindlimb suspension, with or without beta-guanidine propionic acid; soleus muscle analysed |
| Population | Not reported |
| Design | Controlled animal experiment |
| Sample size as reported | 24 rats (8 per group: control, hindlimb suspension, hindlimb suspension plus beta-guanidine propionic acid) |
| Control group | vivarium control rats and untreated hindlimb-suspension rats |
| Route (data field only) | Not reported |
| Endpoints measured | MOTS-C signalling in soleus muscle; AMPK and PGC-1alpha signalling; microRNA-499; soleus fatigue resistance; slow-type fibre percentage; mitochondrial DNA copy number |
| Pathways measured | AMPK signalling · SIRT1 / PGC-1α / biogenesis |
| Research areas | Skeletal muscle · Mitochondrial biology |
| Evidence tier | C — Animal in vivo |
| Publication types | Journal Article, Research Support, Non-U.S. Gov't |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Indirect
- Model applicability
- Mammalian
- Sample size
- Small
- Controls
- Appropriate
- Outcome validity
- Surrogate
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.