Study record
Extension of Mitogenome Enrichment Based on Single Long-Range PCR: mtDNAs and Putative Mitochondrial-Derived Peptides of Five Rodent Hibernators
Emser SV, Schaschl H, Millesi E et al. · Front Genet · 2021
AnalyticalMT-RNR1 / K14Q variantEvidence tier D
What this study reported
Extended a single long-range PCR mitogenome enrichment assay to five hibernating rodents and used the assemblies to predict mitochondrial-derived peptide sequences. Comparison of MOTS-c, SHLP4 and SHLP6 sequences across vertebrate species identified segments of high homology, and candidate OXPHOS amino-acid polymorphisms showed no obvious pattern relating to cold adaptation.
Limitations
Methodological and sequence-prediction study only; MOTS-c peptides were inferred from mtDNA rather than measured, and few species were analysed.
Study record
| Study model | Analytical / methods |
|---|---|
| Substance studied | MT-RNR1 / K14Q variantStudies of the m.1382A>C (K14Q) variant or other MT-RNR1 variation. |
| Species | marmot, ground-squirrel, dormouse |
| System studied | Mitogenomes of five hibernating rodent species enriched by single long-range PCR and sequenced |
| Population | Not reported |
| Design | Sequencing assay development and comparative genomic analysis |
| Sample size as reported | five hibernating rodent species |
| Control group | Not reported |
| Route (data field only) | Not reported |
| Endpoints measured | mitogenome assembly; predicted MOTS-c sequence; SHLP4 and SHLP6 sequences; OXPHOS polymorphisms |
| Pathways measured | Not reported |
| Research areas | Methods · Mitochondrial biology |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Indirect
- Model applicability
- Non-mammalian
- Sample size
- Small
- Controls
- n/a
- Outcome validity
- Exploratory
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.