Study record
The Mitochondrial-Derived Peptide MOTS-c Attenuates Oxidative Stress Injury and the Inflammatory Response of H9c2 Cells Through the Nrf2/ARE and NF-κB Pathways
Shen C, Wang J, Feng M et al. · Cardiovasc Eng Technol · 2022
What this study reported
Reported that hydrogen peroxide lowered viability and superoxide dismutase and raised reactive oxygen species, malondialdehyde and inflammatory cytokines in rat H9c2 cardiomyocytes, and that MOTS-c pretreatment reversed these changes. Knocking down Nrf2 or activating NF-κB reduced the effects of MOTS-c, pointing to the Nrf2/ARE and NF-κB pathways.
Limitations
Single immortalised rat cardiomyoblast line with an acute chemical oxidant challenge and no in vivo work; concentrations tested were supraphysiological relative to circulating levels.
Study record
| Study model | Cell study (in vitro) |
|---|---|
| Substance studied | MOTS-c administeredSynthetic MOTS-c was given to cells, animals or people. |
| Species | rat |
| System studied | Rat H9c2 cardiomyocytes pretreated with MOTS-c before hydrogen peroxide exposure, with Nrf2 knockdown and NF-κB activation models |
| Population | Not reported |
| Design | Cell culture experiments |
| Sample size as reported | Not reported |
| Control group | untreated and hydrogen-peroxide-only cells, with sh-Nrf2 and PMA comparison conditions |
| Route (data field only) | in-culture |
| Endpoints measured | cell viability; reactive oxygen species; malondialdehyde and superoxide dismutase; Nrf2, HO-1 and NQO-1 expression; NF-κB p65 phosphorylation; TNF-α, IL-6 and IL-1β |
| Pathways measured | NRF2 / antioxidant response · Inflammation · Oxidative stress |
| Research areas | Cardiovascular · Inflammation & immunity |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article, Research Support, Non-U.S. Gov't |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Cellular
- Sample size
- Not reported
- Controls
- Appropriate
- Outcome validity
- Surrogate
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.