Study record
Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via APPL1-SIRT1-PGC-1α
Guo Q, Chang B, Yu QL et al. · Diabetologia · 2020
What this study reported
Reported that plasma and skeletal muscle MOTS-c levels were lower in adiponectin-knockout mice and in high-fat-diet mice, and higher after exercise or after administered adiponectin or MOTS-c. In C2C12 myotubes, adiponectin raised MOTS-c expression, and this increase was suppressed when SIRT1 or PGC-1α was inhibited or knocked down.
Limitations
Single mouse strain with no stated sex or randomisation details; mechanistic work rests on one myotube line, and the proposed pathway is inferred from inhibitor and knockdown experiments rather than tissue-specific genetic models.
Study record
| Study model | Animal study (in vivo) |
|---|---|
| Substance studied | SeveralMore than one of these is central to the paper (common for reviews). |
| Species | mouse |
| System studied | Wild-type C57BL/6 mice on a high-fat diet with exercise, adiponectin-knockout mice, and C2C12 myotubes |
| Population | Not reported |
| Design | Controlled animal experiment with cell culture experiments |
| Sample size as reported | Not reported |
| Control group | Not reported |
| Route (data field only) | intraperitoneal |
| Endpoints measured | plasma and muscle MOTS-c levels; APPL1 expression; SIRT1 and PGC-1α expression; insulin resistance |
| Pathways measured | SIRT1 / PGC-1α / biogenesis · Insulin & glucose handling · AMPK signalling |
| Research areas | Metabolic · Skeletal muscle · Exercise |
| Evidence tier | C — Animal in vivo |
| Publication types | Journal Article, Research Support, Non-U.S. Gov't |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Mammalian
- Sample size
- Not reported
- Controls
- Appropriate
- Outcome validity
- Surrogate
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.