Study record
Effects of MOTS-c on the mitochondrial function of cells harboring 3243 A to G mutant mitochondrial DNA
Ahn CH, Choi EH, Kong BS et al. · Mol Biol Rep · 2020
CellsSeveralEvidence tier D
What this study reported
In cybrid cells carrying 3243A>G mutant mtDNA, neither synthetic MOTS-c nor stable MOTS-c overexpression significantly changed ATP content, mitochondrial complex mRNA and protein levels, or mitochondrial respiration.
Limitations
Single cybrid cell model; null result may depend on the exposure conditions and mutation studied.
Study record
| Study model | Cell study (in vitro) |
|---|---|
| Substance studied | SeveralMore than one of these is central to the paper (common for reviews). |
| Species | human |
| System studied | Cybrid cells harbouring 3243A>G mutant mtDNA, treated with synthetic MOTS-c or transfected with a MOTS-c expression vector |
| Population | Not reported |
| Design | Cell culture experiments |
| Sample size as reported | Not reported |
| Control group | untreated or non-transfected mutant cybrid cells |
| Route (data field only) | in-culture |
| Endpoints measured | ATP content; mitochondrial complex mRNA and protein; basal and stimulated mitochondrial respiration |
| Pathways measured | Not reported |
| Research areas | Mitochondrial biology |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Direct
- Model applicability
- Cellular
- Sample size
- n/a
- Controls
- Appropriate
- Outcome validity
- Surrogate
- Support for its own conclusion
- Moderate
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.