Study record
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
Kim SJ, Xiao J, Cohen P et al. · J Vis Exp · 2017
CellsRelated mitochondrial peptideEvidence tier D
What this study reported
Described a cell-culture method combining subcellular fractionation and western blotting to quantify activation and translocation of signalling molecules such as ERK after mitochondrial-derived peptide treatment. The abstract notes that signalling mechanisms of MOTS-c and SHLPs had yet to be elucidated and reports no MOTS-c-specific results.
Limitations
Methods video article; the abstract reports no MOTS-c data, and it is unclear which peptides were tested.
Study record
| Study model | Cell study (in vitro) |
|---|---|
| Substance studied | Related mitochondrial peptideThe paper is mainly about another mitochondrial-derived peptide, such as humanin. |
| Species | human |
| System studied | Cell culture subcellular fractionation and western blotting protocol (HEK293 cells per MeSH) |
| Population | Not reported |
| Design | Methods protocol (cell culture) |
| Sample size as reported | Not reported |
| Control group | Not reported |
| Route (data field only) | in-culture |
| Endpoints measured | ERK activation; nuclear translocation of signalling molecules |
| Pathways measured | MAPK signalling · Nuclear translocation |
| Research areas | Methods |
| Evidence tier | D — Cell, tissue or analytical |
| Publication types | Journal Article, Video-Audio Media |
Appraisal
Each dimension is recorded separately and never combined into a single score. A study can be well controlled and still tell you little about people.
- Relevance to MOTS-c
- Peripheral
- Model applicability
- Cellular
- Sample size
- n/a
- Controls
- n/a
- Outcome validity
- Exploratory
- Support for its own conclusion
- n/a
Provenance
- Bibliographic fields retrieved from PubMed E-utilities efetch (title, journal, date, authors, DOI) on 2026-11-11.
- Annotated from the published abstract.
- Editorial review: approved (2026-11-11).
- Classification follows the site methodology; the record describes the study, not what MOTS-c does in people.